abstract：:Recently, Siemens Diagnostics released a new calcium assay (CA_2) based on complex formation of calcium with Arsenazo III dye for use on the three automated, random access ADVIA Chemistry analyzers (1650, 2400, and 1200). We evaluated this method for analytical performance as well as potential interference from gadoli...

journal_title：Journal of clinical laboratory analysis

authors： Datta P,Dasgupta A

更新日期：2009-01-01 00:00:00

abstract：:It is believed that boundary compositions of matrix proteins might play a role in stone formation; however, few proteomic studies concerning matrix proteins in urinary stones have been conducted. In this study, we extracted low molecular weight proteins from calcium oxalate stones and measured their characteristic pat...

journal_title：Journal of clinical laboratory analysis

authors： Chen WC,Lai CC,Tsai Y,Lin WY,Tsai FJ

更新日期：2008-01-01 00:00:00

abstract：:The genetic basis of infertility has received increasing recognition in recent years, particularly with the advent of assisted reproductive technology. It is now becoming obvious that genetic etiology for infertility is an important cause of disrupted spermatogenesis. Y-chromosome microdeletions and abnormal karyotype...

journal_title：Journal of clinical laboratory analysis

authors： Abid S,Maitra A,Meherji P,Patel Z,Kadam S,Shah J,Shah R,Kulkarni V,Baburao V,Gokral J

更新日期：2008-01-01 00:00:00

abstract：:Candida dubliniensis is a recently described pathogenic species which shares many phenotypic features with Candida albicans and therefore, may be misidentified in microbiological laboratories. Because molecular methods can be onerous and unfeasible in routine mycological laboratories with restricted budgets such as th...

journal_title：Journal of clinical laboratory analysis

authors： de Loreto ES,Pozzatti P,Alves Scheid L,Santurio D,Morais Santurio J,Alves SH

更新日期：2008-01-01 00:00:00

abstract：:The objectives of this research were to establish an automatic analysis method for the determination of serum argininosuccinate lyase (ASL) and to investigate the value of serum ASL test in the diagnosis of various liver disorders. According to the chemical reaction catalyzed by ASL, an enzyme-coupled reaction system ...

journal_title：Journal of clinical laboratory analysis

authors： Feng JF,Chen TM,Wen YA,Wang J,Tu ZG

更新日期：2008-01-01 00:00:00

abstract：:The Venereal Disease Research Laboratory (VDRL) test has long been considered the best serological test for the diagnosis of neurosyphilis. The goal of this study was to find out if the Rapid Plasma Reagin (RPR) could be an alternative to the VDRL. Cerebrospinal fluid (CSF) and sera samples from patients in the follow...

journal_title：Journal of clinical laboratory analysis

authors： Castro R,Prieto ES,da Luz Martins Pereira F

更新日期：2008-01-01 00:00:00

abstract：:Quantitation of hemoglobin alpha 2 delta 2 (HbA2) is a basic and confirmatory test in diagnosing the carrier state of beta-thalassemia. The present study was designed to investigate the effect of cigarette smoking on the diagnostic reliability of HbA2. A total of 2,867 (654 smokers and 2,213 never smokers) male subjec...

journal_title：Journal of clinical laboratory analysis

authors： Tarazi IS,Sirdah MM,El Jeadi H,Al Haddad RM

更新日期：2008-01-01 00:00:00

abstract：:Hematological parameters are affected by different factors that include age, sex, smoking, ethnicity, and environmental altitude. It has been justified that each population must establish its own normal reference intervals to be used in clinical assessments and interpretations. Hematological reference intervals for ad...

journal_title：Journal of clinical laboratory analysis

authors： Sirdah MM,Tarazi IS,El Jeadi H,Al Haddad RM

更新日期：2008-01-01 00:00:00

abstract：:Radioimmunoassay (RIA) that involves purification of the analyte by organic solvent extraction is widely used. Although the extraction RIA is reliable when properly validated, it is time consuming and radioactive, we measure urinary total testosterone with a highly sensitive rapid and accurate time-resolved fluorescen...

journal_title：Journal of clinical laboratory analysis

authors： Bao S,Peng Y,Sheng S,Lin Q

更新日期：2008-01-01 00:00:00

abstract：:Our objectives in this study were to compare results obtained by an enzyme immunoassay (EIA) for F-actin antibody (FAA) immunoglobulin G (IgG) to those determined by an indirect fluorescent antibody (IFA) assay for smooth muscle antibody (SMA) IgG, and to determine the prevalence of FAA in patient sera having serologi...

journal_title：Journal of clinical laboratory analysis

authors： Jaskowski TD,Konnick EQ,Ashwood ER,Litwin CM,Hill HR

更新日期：2007-01-01 00:00:00

abstract：:Circulating bilirubin is thought to function as a physiological antioxidant. One of the decomposition products of this process is the biopyrrins, which include two regioisomers: biotripyrrin-a (1,14,15,17-tetrahydro-2,7,13-trimethyl-1,14-deoxy-3-vinyl-16H-tripyrrin-8,12-dipropionic acid) and biotripyrrin-b (1,14,15,17...

journal_title：Journal of clinical laboratory analysis

authors： Ihara H,Matsumoto T,Morita Y,Hirano A,Okada M,Hashizume N,Shioji I,Yoshimura H

更新日期：2007-01-01 00:00:00

abstract：:The presence of high-molecular intestinal ALP (HIALP) overlapping with bone ALP in the alpha(2)beta region has been demonstrated. In this study we evaluated a method of separating HIALP after its conversion into ALP(5) by the action of protease. Serum samples from patients were mixed with protease at a ratio of 5:1 an...

journal_title：Journal of clinical laboratory analysis

authors： Ooi K,Shiraki K,Morishita Y,Nobori T

更新日期：2007-01-01 00:00:00

abstract：:We report nine donations with ABO inconsistency in reverse typing caused by partly or entirely missing antibodies. A and B antigens and antibodies were examined by serological blood typing, and ABO deoxyribonucleic acid (DNA) analyses were performed by sequence-specific priming and sequencing. A B101 allele was demons...

journal_title：Journal of clinical laboratory analysis

authors： Deng ZH,Zeng JQ,Yu Q,Su YQ,Liang YL,Lu L,Zhu WG,Yang BC

更新日期：2007-01-01 00:00:00

abstract：:There is an increasing demand for genetic testing of patients with hypercoagulability. We have developed a multiplex-polymerase chain reaction (PCR) technique that incorporates the state-of-the art of genetic testing for thrombosis mutations (Factor V Leiden [FVL] and G20210A F2 and C46T F12). The sequences are detect...

journal_title：Journal of clinical laboratory analysis

authors： López M,Giraldo P,Alvarez P,Cornudella R,Pocoví M,Martínez A,Fontcuberta J,Soria JM

更新日期：2007-01-01 00:00:00

abstract：:A modification of the existing spectrophotometric kinetic method for the determination of pancuronium bromide (PCBr), based on pooled human serum cholinesterase (ChE, EC 3.1.1.8 acylcholine acylhydrolase) inhibition, was developed. Butyrylthiocholine iodide (concentration 1.667 mmol/L) was used as substrate and determ...

journal_title：Journal of clinical laboratory analysis

authors： Stankov-Jovanovic VP,Nikolic-Mandic SD,Mandic LjM,Mitic VD

更新日期：2007-01-01 00:00:00

abstract：:Apoptosis of epithelial hepatocytes plays a pivotal role in acute as well as in chronic liver diseases. The cleavage of cytokeratin-18 (CK-18) by caspases is an early event in the apoptotic process. We therefore sought to investigate serum levels of CK-18 and 20S proteasome in patients with liver cirrhosis, primary gr...

journal_title：Journal of clinical laboratory analysis

authors： Hetz H,Hoetzenecker K,Hacker S,Faybik P,Pollreisz A,Moser B,Roth G,Hoetzenecker W,Lichtenauer M,Klinger M,Krenn CG,Ankersmit HJ

更新日期：2007-01-01 00:00:00

abstract：:This study was designed to measure the correlation between left ventricular (LV) remodeling 3 months after successful reperfusion therapy, and the levels of serum N-terminal pro-brain natriuretic peptide (NT-proBNP) and high-sensitivity C-reactive protein (hs-CRP) at 3 days after acute myocardial infarction (AMI), and...

journal_title：Journal of clinical laboratory analysis

authors： Xiaozhou H,Jie Z,Li Z,Liyan C

更新日期：2006-01-01 00:00:00

abstract：:Several retrospective studies have shown that a high level of microsatellite instability (MSI-H) is an important prognostic factor of a more favorable outcome in stage II and III colorectal cancer (CRC) patients. In this study, three commonly used polymerase chain reaction (PCR)-based MSI analysis techniques were comp...

journal_title：Journal of clinical laboratory analysis

authors： Deschoolmeester V,Baay M,Wuyts W,Van Marck E,Pelckmans P,Lardon F,Vermorken JB

更新日期：2006-01-01 00:00:00

abstract：:It is well established that the targeted receptor for ciguatoxin (CTX) in mammalian tissues is the sodium channel, affecting the influx of sodium into cells and altering the action potential and function of the cell. Since the syntheses of fragments of CTX has become available, our focus has been on the receptor funct...

journal_title：Journal of clinical laboratory analysis

authors： Hokama Y,Chun KE,Campora CE,Higa N,Suma C,Hamajima A,Isobe M

更新日期：2006-01-01 00:00:00

abstract：:Enteroviruses (EVs) are the most commonly identified cause of aseptic meningitis. Rapid detection and characterization of EV meningitis is essential in making decisions for patient management and treatment. A total of 52 cases of acute aseptic meningitis that occurred from March 2003 to April 2005 were investigated fo...

journal_title：Journal of clinical laboratory analysis

authors： Dumaidi K,Frantzidou F,Papa A,Diza E,Antoniadis A

更新日期：2006-01-01 00:00:00

abstract：:N-terminal pro-brain natriuretic peptide (NT-proBNP) and BNP measurement could have a significant role in differentiating dyspnea between cardiac or pulmonary origin in the emergency room. The development of new and different commercial assays for these B-type natriuretic peptides offers the possibility of improving a...

journal_title：Journal of clinical laboratory analysis

authors： Sanz MP,Borque L,Rus A,Vicente B,Ramírez Y,Lasa L

更新日期：2006-01-01 00:00:00

abstract：:It has been proposed that sample storage may have some influence on the parameters of oxidative stress status (OSS) in biological fluids. We measured four important OSS parameters in plasma of 23 healthy subjects and repeated the measurements in the same samples kept at -70 degrees C after different time intervals. Hy...

journal_title：Journal of clinical laboratory analysis

authors： Firuzi O,Mladenka P,Riccieri V,Spadaro A,Petrucci R,Marrosu G,Saso L

更新日期：2006-01-01 00:00:00

abstract：:Preeclampsia continues to be a major cause of maternal and perinatal mortality and morbidity worldwide. The etiopathogenesis of preeclampsia is not fully understood. Neopterin and interleukin-2 (IL-2) production reflects cellular immunity. Our purpose was to determine the levels of neopterin and interleukin-2 receptor...

journal_title：Journal of clinical laboratory analysis

authors： Kaleli I,Kaleli B,Demir M,Yildirim B,Cevahir N,Demir S

更新日期：2005-01-01 00:00:00

abstract：:Urinary proteins from six patients with esophageal cancer and two with stomach cancer were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Analyses were performed on days-1 to 3, 5, 7, 10, 14, and 21 (or 22) after surgery. The protein patterns were scanned by densitometry and divided ...

journal_title：Journal of clinical laboratory analysis

authors： Yokomizo K,Iijima S,Sakai N,Kurihara Y,Hitratsuka N,Nagai K,Iwai T,Shiba K

更新日期：2005-01-01 00:00:00

abstract：:The Brazilian External Quality Assessment Program in Immunohematology (BEQAPI) was introduced with the objective of evaluating the quality of diagnosis in immunohematology. From 1992 to 2003, proficiency tests for ABO grouping, Rh (D, C, c, E, e), K phenotyping, direct antiglobulin testing (DAT), antibody screening (A...

journal_title：Journal of clinical laboratory analysis

authors： Melo L,Pellegrino J Jr,Bianco C,Castilho L

更新日期：2005-01-01 00:00:00

abstract：:A novel severe acute respiratory syndrome-associated coronavirus (SARS-CoV) has been discovered. The detection of both antigens and antibodies in SARS-CoV from human specimens with suspected SARS plays an important role in preventing infection. We developed a novel rapid immunochromatographic test (RICT) based on the ...

journal_title：Journal of clinical laboratory analysis

authors： Kogaki H,Uchida Y,Fujii N,Kurano Y,Miyake K,Kido Y,Kariwa H,Takashima I,Tamashiro H,Ling AE,Okada M

更新日期：2005-01-01 00:00:00

abstract：:The purpose of this study was to evaluate whether IL-1 beta (IL-1beta promoter and IL-1beta exon 5), IL-1 receptor antagonist (IL-1 Ra), and IL-4 (IL-4 promoter and IL-4 intron 3) gene polymorphisms act as markers of susceptibility to Kawasaki disease (KD), or of the severity of the disease. The study included 107 KD ...

journal_title：Journal of clinical laboratory analysis

authors： Wu SF,Chang JS,Wan L,Tsai CH,Tsai FJ

更新日期：2005-01-01 00:00:00

abstract：:Rare abnormal haemoglobin (Hb) often poses a problem in the diagnosis of relative electrophoresis mobility. The rare neutral Hbs interact with the Hb S and thus can increase the severity of sickle cell anaemia. In the present study, we investigated the use of reversed-phase high-performance liquid chromatography (RP-H...

journal_title：Journal of clinical laboratory analysis

authors： Yapo PA,Datté JY,Yapo A,Wachman H

更新日期：2004-01-01 00:00:00

abstract：:We aimed to evaluate whether a PEG-IAT method, modified for gel technique, could replace the two-stage enzyme method for routine identification of red blood cell (RBC) antibodies.A total of 161 selected patient samples were analyzed with the two-stage enzyme tube technique and the PEG-IAT in gel techniques. In 65 samp...

journal_title：Journal of clinical laboratory analysis

authors： Gustafsson L,Wikman A,Lundahl J

更新日期：2004-01-01 00:00:00

abstract：:The two serum proteins of the complement cascade in the highest concentrations, C3 and C4, respond to various conditions in much the same manner as do other positive acute-phase proteins. A major difference is that they are relatively sluggish in response to cytokine drive, requiring several days rather than hours to ...

journal_title：Journal of clinical laboratory analysis

authors： Ritchie RF,Palomaki GE,Neveux LM,Navolotskaia O,Ledue TB,Craig WY

更新日期：2004-01-01 00:00:00

abstract：:Clinicians recognize and compensate for limitations in estimating the glomerular filtration rate (GFR) using serum creatinine (sCr) measurements by the use of timed collections and mathematical manipulations of sCr. These limitations stem from that fact that sCr is affected by nonrenal influences, including muscle mas...

journal_title：Journal of clinical laboratory analysis

authors： Massey D

更新日期：2004-01-01 00:00:00

abstract：:Hemoglobin F (HbF) is an effective inhibitor of HbS polymerization. Hydroxyurea (HU) is used to increase HbF synthesis and improve the clinical course of sickle cell disease (SCD) patients. We studied a series of laboratory parameters concerning HbF production and reticulocyte response, and compared data between two g...

journal_title：Journal of clinical laboratory analysis

authors： Borba R,Lima CS,Grotto HZ

更新日期：2003-01-01 00:00:00

abstract：:Alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH), which are most abundant in the liver, are the main enzymes involved in ethanol metabolism in humans. Gender-related differences in total liver ADH and ALDH activity among different animal species have been observed in many studies. We measured total ADH an...

journal_title：Journal of clinical laboratory analysis

authors： Chrostek L,Jelski W,Szmitkowski M,Puchalski Z

更新日期：2003-01-01 00:00:00

abstract：:The aim of the present study was to investigate the serum total protein (TP), total sialic acid (TSA), lipid-associated sialic acid (LSA), LSA/TP, and LSA/TP values in type 2 diabetes mellitus (DM) patients. Two study groups (healthy controls and type 2 DM subjects) were examined. For the type 2 DM group, 120 patients...

journal_title：Journal of clinical laboratory analysis

authors： Ekin S,Meral I,Gunduz H,Mert N

更新日期：2003-01-01 00:00:00

abstract：:Congenital heart disease (CHD) is a common cardiac defect found in infants and children. Despite advances in diagnosis and treatment, our understanding of the causative mechanism and etiology of CHD is limited. To determine the genetic etiology of CHD, we selected 11 consecutive short tandem-repeat polymorphic (STRP) ...

journal_title：Journal of clinical laboratory analysis

authors： Shi YR,Hsieh KS,Wu JY,Lee CC,Tsai CH,Yu MT,Chang JS,Tsai FJ

更新日期：2003-01-01 00:00:00

abstract：:Hypoxia and ischemia play important roles in the onset and progression of glaucoma. Insulin-like growth factors (IGF) are important neurotrophic agents that respond to hypoxia-ischemia. In this study, we enrolled 60 primary open angle glaucoma (POAG) patients and 104 healthy volunteers from the China Medical College H...

journal_title：Journal of clinical laboratory analysis

authors： Tsai FJ,Lin HJ,Chen WC,Chen HY,Fan SS

更新日期：2003-01-01 00:00:00

abstract：:The proteoglycan decorin inhibits TGF-beta; therefore, it could antagonize progression of fibrotic diseases associated with activation of TGF-beta(1). The effect of decorin transfection in human mesangial cells (HMCs) on the expression of genes related to kidney fibrosis was investigated. HMCs, isolated from glomeruli...

journal_title：Journal of clinical laboratory analysis

authors： Costacurta A,Priante G,D'Angelo A,Chieco-Bianchi L,Cantaro S

更新日期：2002-01-01 00:00:00

abstract：:The objective of this study was to analyze apparently discrepant results that arose during the use of an indirect immunofluorescence (IIF) assay using transfected HEp-2 cells to detect anti-SS-A/Ro autoantibodies in human sera. Fourteen sera that had SS-A/Ro antibodies as detected on this commercial substrate, but did...

journal_title：Journal of clinical laboratory analysis

authors： Fritzler MJ,Hanson C,Miller J,Eystathioy T

更新日期：2002-01-01 00:00:00

abstract：:The aim of this study was to evaluate anti-HEV antibody profiles in urine specimens in comparison to corresponding serum samples to assess the utility of urine as a clinical specimen. Paired serum and urine specimens from 71 hepatitis E patients, 33 non-E hepatitis patients, 63 patients with nonhepatic diseases, and 2...

journal_title：Journal of clinical laboratory analysis

authors： Joshi MS,Walimbe AM,Arankalle VA,Chadha MS,Chitambar SD

更新日期：2002-01-01 00:00:00

abstract：:The pathogenic role of immune-mediated mechanisms in chronic hepatitis C virus (HCV) infection has not yet been elucidated. In this study, we report different cytokine expression profiles from hemodialysis (HD) and non-HD HCV (+) patients. IL-1beta, IL-2, IL-4, IL-6, TNF-alpha, and TGF-beta1 serum levels, and liver bi...

journal_title：Journal of clinical laboratory analysis

authors： Spanakis NE,Garinis GA,Alexopoulos EC,Patrinos GP,Menounos PG,Sklavounou A,Manolis EN,Gorgoulis VG,Valis D

更新日期：2002-01-01 00:00:00